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books
| book details |
The Immunological Synapse Part C: Volume 193
Volume editor Clément Thomas, Volume editor Lorenzo Galluzzi
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normal price: R 6 781.95
Price: R 6 103.95
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| book description |
The Immunological Synapse - Part C, Volume 193 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand γδ T cells and evaluate their cytotoxicity.
| product details |
Normally shipped |
Publisher | Elsevier Science Publishing Co Inc
Published date | 4 Feb 2025
Language |
Format | Hardback
Pages | 280
Dimensions | 235 x 191 x 0mm (L x W x H)
Weight | 580g
ISBN | 978-0-4432-1868-2
Readership Age |
BISAC | science / life sciences / cytology
| other options |
Normally shipped |
Readership Age |
Normal Price | R 7 477.95
Price | R 6 729.95
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